Just a reminder today that Nutanix does not support USB passthrough on all platforms/hypervisors. There are no plans to support USB passthrough on Nutanix platforms due to security concerns and due to the fact that it affects features such as live migration, HA, 1-Click upgrades and more.
If you need a guest VM to access USB devices, using AnywhereUSB is a valid solution. This solution uses a USB hub accessible over TCP/IP. A driver is installed on the VM and it emulates a USB device connected to the hub as if it was directly attached to the VM. This method is well-known to work and does not affect the features mentioned above. Please see the Digi site for further details: